P050 – The Use of the RapidINTEL™ Plus Kit and the RapidHIT™ ID System to Analyse Contact Traces Secured on SceneSafe™ Adhesive Tape.

11:13

Wednesday August 19^th

Station 10

Duration: 12 minutes

03. Human identification

Tomasz Kupiec

Contact traces collected from surfaces and clothing at crime scenes are a valuable source of genetic information in forensic investigations. Due to the low DNA yield and the frequent presence of mixtures, these samples pose a challenge, both during testing and when analysing the results. The RapidHIT™ ID System, together with RapidINTEL™ Plus analysis cartridges, enables the testing of biological traces from crime scenes in under 90 minutes.

The aim of this study was to assess the suitability of the RapidINTEL™ Plus kit for the analysis of contact traces collected using SceneSafe™ adhesive tapes. The sampling and analysis method developed during the study was designed to simplify the sampling process as much as possible whilst maintaining compatibility with the rapid DNA platform and the software.

35 contact traces were collected using SceneSafe™ tape from surfaces commonly encountered in forensic practice, such as mobile phones, keyboards, mice and worn clothing. Using a manual sampling punch, circular foil fragments with a diameter of 5 mm were collected; three of these were placed in a cartridge and analysed. The samples were examined using the RapidINTEL™ Plus kit and the Specialized program, designed for samples with low DNA content. The completeness of the profiles obtained and the peak balance were assessed. In addition, the sensitivity of the method was determined using the NIST SRM 2372a DNA concentration standard and K007 control. It was estimated that the amount of 1 ng DNA input is the limit that allows obtaining a complete genetic profile of the trace.

The results obtained indicate that the combined use of SceneSafe™ tape and the RapidINTEL™ Plus kit enables the generation of usable STR profiles from around 70% of contact traces. It was observed that the effectiveness of the analysis is influenced by the type of surface and the conditions under which the traces were deposited. Higher efficiency was observed on non-porous surfaces, such as plastic, glass or metal surfaces of electronic devices, whereas stochastic effects characteristic of trace analyses with low DNA content were more frequently observed in the case of fabrics.

The proposed approach offers a practical solution for the rapid processing of contact traces, both in the laboratory and, potentially, at the crime scene. Its application could significantly enhance the ability to obtain rapid investigative leads based on contact DNA traces.

Authors

Tomasz Kupiec (Institute of Forensic Research, Poland)
Patrycja Roszak (Military Gendarmerie Training Centre, Poland)
Andrzej Doniec (Institute of Forensic Research, Poland)

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P050 – The Use of the RapidINTEL™ Plus Kit and the RapidHIT™ ID System to Analyse Contact Traces Secured on SceneSafe™ Adhesive Tape.

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