P129 – A Portable LAMP-microfluidic Point-of-care System for Rapid Human DNA Detection and Sex Identification

16:07

Wednesday August 19^th

Station 10

Duration: 12 minutes

03. Human identification

Yazi Zheng

Rapid identification of human DNA and sex determination is essential for forensic investigations, particularly for timely screening at crime scenes. However, conventional PCR-based methods remain limited by processing time, complex instrumentation, and poor suitability for rapid on-site analysis. Here, we develop a multiplex loop-mediated isothermal amplification (LAMP) system targeting the Cyt b and SRY genes for simultaneous human DNA detection and sex identification. The system was integrated into a rapid detection workflow combining simplified sample lysis, multiplex amplification, and a microfluidic platform, with reagents prepared as lyophilized beads to enhance field deployability and storage stability. The entire workflow, from sample processing to result output, was completed in 35 min, enabling rapid and user-friendly point-of-care testing. The LAMP system achieved a sensitivity as low as 25 pg and demonstrated strong analytical robustness, including reliable detection of trace male DNA in mixed samples. Validation with diverse clinical body fluid specimens showed 100% accuracy. Furthermore, testing of touch DNA from both simulated and real samples achieved a detection rate of 62.5%. Together, the multiplex LAMP system provides a rapid, sensitive, and portable strategy for human DNA detection and sex identification. The combination of lyophilized reagents and microfluidic integration highlights its strong potential for rapid on-site forensic applications.

Authors

Yazi Zheng (Department of Forensic Genetics, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, China)
Shengqiu Qu (Department of Forensic Genetics, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, China)
Xiameng Chen (Department of Forensic Genetics, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, China)
Qiuyun Yang (Department of Forensic Genetics, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, China)
Peng Bai (Department of Forensic Genetics, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, China)
Meili Lv (Department of Forensic Genetics, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, China)
Lin Zhang (Department of Forensic Genetics, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, China)
Weibo Liang (Department of Forensic Genetics, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, China)

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P129 – A Portable LAMP-microfluidic Point-of-care System for Rapid Human DNA Detection and Sex Identification

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