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P242 – From Touch to Trace: Optimizing DNA Yield from Latent Fingerprints

10:37

Thursday August 20^th

Station 17

Duration: 12 minutes

04. Forensic biology

Monica Omedei

The integration of fingerprint examination and DNA analysis is a critical component of modern forensic investigations, particularly in the context of the increasing use of touch DNA evidence. The recovery of genetic material from latent fingerprints offers the potential to associate ridge detail evidence with DNA profiles, thereby enhancing individual identification. However, the chemical and physical processes routinely applied for fingermark visualization may adversely affect the integrity and recoverability of biological material, with potential consequences for downstream genetic analysis.

This study aims to evaluate the impact of commonly used fingermark development techniques on DNA recovery in order to support the optimization of forensic workflows. Latent fingerprints were deposited under controlled conditions on two non-porous substrates, glass and plastic, with five fingermarks applied to each surface. The samples were processed using three widely employed visualization methods: cyanoacrylate fuming, Lumicyano, and Small Particle Reagent (SPR). Following development, DNA was collected and extracted using standardized protocols routinely applied in forensic laboratories.

The performance of each technique was assessed in terms of DNA yield and compatibility with subsequent forensic genetic analysis. The comparative evaluation provides practical insight into the suitability of specific fingermark development methods according to surface type and investigative priorities. The results contribute to defining an optimized operational approach that balances effective fingerprint visualization with maximized DNA recovery, reinforcing the combined evidential value of dactyloscopic and genetic analyses in routine forensic casework.

Authors

Monica Omedei (Centro Avanzato di Diagnostica “A. Bertinaria”, Italy)
Selena Cisana (Centro Avanzato di Diagnostica “A. Bertinaria”, Italy)
Vincenzo Agostini (Department of Science, Technology and Innovation (DISIT), University of Eastern Piedmont, Italy)
Paolo Garofano (Centro Avanzato di Diagnostica “A. Bertinaria”, Italy)
Gabriele Rotter (Postgraduate School of Legal Medicine, BIOMORF Department, University of Messina, Italy)

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P242 – From Touch to Trace: Optimizing DNA Yield from Latent Fingerprints

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