P282 – State DNA Database of Victims of the War and the Dictatorship in Spain. Validation of the Bonaparte Software by the INTCF.

10:25

Friday August 21^st

Station 08

Duration: 12 minutes

03. Human identification

Pedro A. Barrio

Background

The recovery and identification of skeletal human remains related to the Spanish Civil War (1936-1939) and the Dictatorship (1939-1975) is a complex process involving scientific associations, as well as private and public laboratories, with the participation of family members.

With the enactment of Spain’s Democratic Memory Law (Law 20/2022, of 19 October), an important step forward was taken through the creation of the State DNA Database of Victims of the War and the Dictatorship (BEADN, regulated in Article 23). This database is under the responsibility of the National Institute of Toxicology and Forensic Sciences (INTCF), a public forensic science agency attached to the Spanish Ministry of Justice.

Aim

In this poster, we present the validation of the Bonaparte software by the INTCF, which will be the management software of the BEADN.

Material and methods

To perform this validation, a mock data panel was used, consisting of genetic profiles (for A‑STRs, Y‑STRs, and mtDNA markers) from 71 unidentified remains (UI) and 55 putative relatives (FM), distributed across 42 pedigrees (PT). These UIs and FMs were allocated among 3 hypothetical laboratories tasked with the identification of the analyzed remains.

Bonaparte software version 4.2‑r24_b1,275 was employed. Repeatability, reproducibility, and concordance were assessed. Concordance was evaluated qualitatively using CODIS 9 and quantitatively using Familias v3.3.1, applying a 30% coefficient‑of‑variation threshold and the Wilcoxon signed‑rank test.

Results and conclusions

The results show 100% repeatability, reproducibility and qualitative concordance. Regarding quantitative concordance, only one case exceeded the 30% coefficient‑of‑variation threshold, as a consequence of the low LR value obtained; however, the overall differences were not statistically significant.

These findings support the suitability of the Bonaparte software for managing the Spanish BEADN and open a new pathway for the re‑signification of the victims of the Civil War and the Dictatorship in Spain. Once the regulation governing the BEADN is approved, all accredited laboratories will be trained and granted access to register profiles and make comparisons in the system.

Authors

Pedro A. Barrio (National Institute of Toxicology and Forensic Sciences. Department of Madrid. Service of Biology., Spain)
Mª José Farfán (National Institute of Toxicology and Forensic Sciences. Department of Madrid. Service of Biology., Spain)
Miguel Paredes (Sciences. Department of Barcelona. Service of Biology., Spain)
Ángel Serrano (National Institute of Toxicology and Forensic Sciences. Department of Barcelona. Service of Biology., Spain)
Victoria Prieto (National Institute of Toxicology and Forensic Sciences. Department of Sevilla. Service of Biology., Spain)
Carlos Baeza (National Institute of Toxicology and Forensic Sciences. Department of Sevilla. Service of Biology., Spain)

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P282 – State DNA Database of Victims of the War and the Dictatorship in Spain. Validation of the Bonaparte Software by the INTCF.

Background

Aim

Material and methods

Results and conclusions

Authors

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